IMPORTANT TERMS:
· A site (Aminoacyl site): A functional site in larger subunit of ribosome where tRNA carrying amino acid gets attached.
·
Anticodon:
Three base sequences present on tRNA that are complementary to the triplet code
of mRNA.
·
Bacteriophages:
Viruses that infect the bacteria.
·
Central
Dogma: The unidirectional flow of information form
DNA—>RNA—> Protein
·
Codon:
A group of three nucleotide bases on mRNA that specify a particular amino acid
in protein synthesis.
·
DNA
Ligase: Enzyme that joins okazaki fragments to form lagging
strand.
·
DNA
Polymerase: Enzyme that link free DNA nucleotides
to form the complementary strand. It works in 5’-3’ direction.
·
Exons:
Regions of a eukaryotic gene that contain the information necessary to form a
protein.
·
Frameshift
Mutation: Mutation caused due to insertion or deletion of a
single base that shifts the entire reading frame of DNA.
·
F-Factor:
Fertility factor or sex factor present in the plasmid of some bacteria. F+
is male bacterium and F- is female bacterium.
·
Genetic
Code: The genetic information in the form of sequences of
base triplets in DNA which controls the sequence of amino acids in a protein.
·
Helicases:
Enzyme
that help in unwinding the two strand of DNA.
·
Hfr
Strain (High frequency recombination strain): A male
bacterium having fertility factor (F-factor) integrated to its chromosome.
·
Inducer:
The chemical that induces or turns the switch on of an operon to produce the
enzyme.
·
Inducible
Enzymes: Enzymes whose production is induced or stimulated
by a compound called inducer.
·
Introns:
Regions
of a eukaryotic gene that do not have information for a protein.
·
Operon:
A part of genetic material which acts as a single regulated unit having
structural gene/genes and regulatory genes.
·
P-site
(Peptidyl Site): A functional site in larger subunit of
ribosome where tRNA from A site is pulled by the process of translocation.
·
Primer:
Short segments of RNA formed on DNA template before the replication begins.
·
Proto-oncogenes:
Potential oncogenes present in the normal cells before they are triggered.
·
RNA
Polymerase : Enzyme that catalyses the formation of
RNA from one strand of DNA.
·
Repressor
enzymes: Enzymes whose production is repressed or stopped by
the end product of their metabolism.
·
Reverse
Transcription: Transcription of double stranded DNA
from single stranded RNA with the help of enzyme reverse transcriptase.
·
Split
Genes: Eukaryotic genes consisting of coding (exons) and
non-coding (introns) segments in DNA.
·
Topoisomerases:
Enzymes that break and reseal one strand of DNA.
·
Transcription:
The
process of synthesis of RNA from one of the strands of DNA.
·
Transformation:
The phenomenon by which DNA isolated from one type of cells when introduced
into another type is able to bestow (give) some of the properties of the former
to the latter.
·
Translation:
To change the coded message of mRNA into the sequence of amino acids.
·
Translocation:
The process in which a tRNA molecule
moves from A site to P site due to movement of ribosome on mRNA during
protein synthesis.
·
Wobble
Position: Position of third base in a codon, which even after
mutation permits binding of anticodon of the corresponding tRNA.
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